Maudu'ai da Hanyoyi:

• Mahalarta:Jimlar158 lafiya maza da mata masu shekaru tsakanin20 da 59 shekaruaka dauka aiki. Masu ba da agaji tare da tarihin likita mai tsanani ko na yau da kullun, waɗanda suka cinye fiye da500 ml na kofi, shayi, abubuwan sha masu laushi, da abubuwan sha a kullum, wadandaya sha giya fiye da kwana biyu a mako, waɗanda suka yi amfani da kariyar antioxidant akai-akai a bayawatanni uku, masu shan taba, waɗanda ke da halayen motsa jiki mai tsanani, kuma waɗanda ba su cika ka'idodin amfani da yau da kullun na 500 - 2500 ml na ruwa mai tsafta ba an cire su. Daga ƙarshe, mahalarta 41 masu cancanta an ba su bazuwar zuwa rukunin ruwa na hydrogen (n = 22) da ƙungiyar ruwa ta al'ada (n = 19). Duk da haka, yayin aikin binciken, mahalarta 2 sun fita daga rukunin ruwa na hydrogen da 1 daga rukunin ruwa na al'ada. Daga karshe,20mahalarta a cikin rukunin ruwa na hydrogen da18a cikin rukunin ruwa na al'ada sun kammala gwajin sa baki na mako 4.
• Hanyoyin shiga tsakani:Ƙungiyar ruwa ta hydrogen ta cinye1.5 lita na ruwa mai arziki a hydrogen(tare da ƙwayar hydrogen na 0.753 ± 0.012 mg / l) kowace rana, yayin da ƙungiyar ruwa ta al'ada ta sha daidai adadin ruwa na al'ada. Ana buƙatar mahalarta su gama ruwan a cikin kwalban 500 ml a cikin sa'a daya bayan bude shi. Bayan kofi, shayi, abubuwan sha masu laushi, da abubuwan sha, ba a yarda da wani ƙarin ruwa ba, kuma an kula da yawan shan waɗannan ƙarin abubuwan sha bai wuce 500 ml a kowace rana ba.

• Ƙarfin Antioxidant da Lalacewar Oxidative: Bayan makonni hudu, duka shan ruwan al'ada da ruwa mai wadatar hydrogen sun haifar da karuwa a cikin karfin maganin antioxidant na kwayoyin halitta (BAP). A cikin yawan jama'a, babu wani muhimmin bambanci tsakanin rukunin ruwa na hydrogen da ƙungiyar ruwa ta al'ada dangane da BAP. Duk da haka, ga mahalarta fiye da shekaru 30, shan ruwan hydrogen ya haifar da karuwa mai yawa a BAP (p = 0.028). A cikin ƙaramin rukuni (< 30 shekaru), ba a lura da tasirin ruwa na hydrogen akan BAP ba (p = 0.534).
• Apoptosis na Peripheral Blood Mononuclear Cells (PBMCs) da Profile na Jini Immune Yawan Jama'a:A asali, babu wani bambanci mai mahimmanci a cikin yawan ƙwayoyin apoptotic a cikin jini tsakanin ƙungiyoyin biyu. Koyaya, bayan gwajin makonni 4, idan aka kwatanta da rukunin ruwa na al'ada, adadin PBMCs apoptotic a cikin rukunin ruwa na hydrogen ya ragu sosai (p = 0.036). Bugu da ƙari, ta hanyar nazarin cytometry mai gudana, an gano cewa yawan adadin CD14+ a cikin rukunin ruwa na hydrogen ya ragu.
• Nazari na Rubutu:Binciken jerin abubuwan RNA na PBMC ya nuna cewa akwai manyan bambance-bambance tsakanin rubutun rukunin ruwa na hydrogen da na rukunin ruwa na al'ada. Mafi mahimmanci, cibiyoyin sadarwar da ke da alaƙa da amsawar kumburi da kuma hanyar siginar NF-κB a cikin rukunin ruwa na hydrogen sun ragu sosai.